2XXW

Crystal structure of the GluK2 (GluR6) D776K LBD dimer in complex with glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Detector	RAYONIX
Spacegroup name	I 2 2 2
Unit cell lengths	93.687, 106.821, 112.854
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.974 - 2.300
R-factor	0.1936
Rwork	0.191
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xxr
RMSD bond length	0.004
RMSD bond angle	0.762
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.060	0.580
Number of reflections	25332
<I/σ(I)>	13.2	2
Completeness [%]	99.2	98.3
Redundancy	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP 21% PEG 4000, 80MM SODIUM ACETATE
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP 21% PEG 4000, 80MM SODIUM ACETATE