2XXT
Crystal structure of the GluK2 (GluR6) wild-type LBD dimer in complex with kainate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 97.285, 105.915, 113.478 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.724 - 1.900 |
| R-factor | 0.1741 |
| Rwork | 0.172 |
| R-free | 0.20950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xxr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.056 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.730 |
| Number of reflections | 44830 | |
| <I/σ(I)> | 12.7 | 2.8 |
| Completeness [%] | 96.5 | 98.7 |
| Redundancy | 5.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP 23% PEG 4000, 9% PROPAN-2-OL, 80MM SODIUM ACETATE. |
