2XXA
The Crystal Structure of the Signal Recognition Particle (SRP) in Complex with its Receptor(SR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2009-09-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.100, 131.040, 266.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.300 - 3.940 |
| R-factor | 0.2334 |
| Rwork | 0.232 |
| R-free | 0.26690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1HQ1 AND 1RJ9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 4.170 |
| High resolution limit [Å] | 3.940 | 3.940 |
| Rmerge | 0.040 | 0.670 |
| Number of reflections | 26790 | |
| <I/σ(I)> | 12.9 | 1.6 |
| Completeness [%] | 94.0 | 95.3 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | 12% PEG 2000 MME, 260MM LICL, 50MM MGOAC2, 11% (W/V) GLYCEROL, 50 MM BIS-TRIS PH 6.9 |
