2XWX
Vibrio cholerae colonization factor GbpA crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.955, 120.182, 67.177 |
| Unit cell angles | 90.00, 108.41, 90.00 |
Refinement procedure
| Resolution | 19.940 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.24600 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.124 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.330 |
| Number of reflections | 73728 | |
| <I/σ(I)> | 14.4 | 3 |
| Completeness [%] | 96.1 | 91.6 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M MG(HCO3)2, 50% (W/V) PEG 3350, 3.33% (W/V) D-SORBITOL, PH 7.5 |
