2XWQ
Anaerobic cobalt chelatase from Archeaoglobus fulgidus (CbiX) in complex with metalated sirohydrochlorin product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.010, 99.000, 116.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.570 - 2.010 |
| R-factor | 0.21979 |
| Rwork | 0.217 |
| R-free | 0.28234 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dj5 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.384 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.300 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.010 |
| Rmerge | 0.150 | 0.620 |
| Number of reflections | 253190 | |
| <I/σ(I)> | 8.2 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | pH 6.8 |
