2XWJ
Crystal Structure of Complement C3b in Complex with Factor B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-12 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 262.160, 297.870, 341.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.757 - 4.000 |
| R-factor | 0.2301 |
| Rwork | 0.228 |
| R-free | 0.28080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PARTIAL MODEL FROM 2XWB |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.357 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 4.220 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.160 | 0.460 |
| Number of reflections | 104810 | |
| <I/σ(I)> | 7.2 | 3.2 |
| Completeness [%] | 93.4 | 79 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | pH 4.0 |
