2XWC
Crystal structure of the DNA binding domain of human TP73 refined at 1.8 A resolution
Replaces: 2XIPExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 110.370, 110.370, 110.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.360 - 1.820 |
| R-factor | 0.1909 |
| Rwork | 0.189 |
| R-free | 0.22824 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 20CJ |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.542 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.360 | 49.360 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.160 | 1.240 |
| Number of reflections | 280763 | |
| <I/σ(I)> | 11.9 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.2 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.2M SODIUM POTASSIUM TARTRATE, 0.25% PEG MME 5000, 0.1M TRIS PH 9 |
