2XVC
Molecular and structural basis of ESCRT-III recruitment to membranes during archaeal cell division
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.240, 65.240, 60.992 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.500 - 2.150 |
| R-factor | 0.22986 |
| Rwork | 0.229 |
| R-free | 0.25425 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.990 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 8461 | |
| <I/σ(I)> | 24 | 5.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 10.7 | 20.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 15% PEG 4000, 5 MM CDCL2, PH 4 |
