2XTL
Structure of the major pilus backbone protein from Streptococcus Agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.863, 104.681, 159.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.750 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.21588 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x9w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.048 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.100 | 0.600 |
| Number of reflections | 108305 | |
| <I/σ(I)> | 14.9 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.6 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 25% PEG4K, 0.1M HEPES PH 7.2, 90 MM POTASSIUM SODIUM TARTRATE |
