2XSW
Crystal structure of human INPP5E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-09-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.528, 103.579, 137.545 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.468 - 1.900 |
| R-factor | 0.1665 |
| Rwork | 0.165 |
| R-free | 0.19470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.470 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 56918 | |
| <I/σ(I)> | 7.6 | 0.9 |
| Completeness [%] | 98.5 | 97.3 |
| Redundancy | 6.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PROTEIN WAS INCUBATE WITH 1MM PHOSPHATIDYLINOSITOL-3,4,5-TRIPHOSPHATE AND 2MM MGCL2 PRIOR SETTING-UP THE PLATES. PROTEIN WAS THEN CRYSTALLIZED AGAINST 0.2M MGCL2, 10% W/V PEG8K AND 0.1M TRIS PH 7. |
