2XSQ
Crystal structure of human Nudix motif 16 (NUDT16) in complex with IMP and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-08 |
| Detector | ADSC CCD |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 141.583, 141.583, 141.583 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.740 - 1.720 |
| R-factor | 0.15983 |
| Rwork | 0.159 |
| R-free | 0.18145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cou |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.276 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.690 | 1.810 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.060 | 0.700 |
| Number of reflections | 25010 | |
| <I/σ(I)> | 11.3 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS MIXED WITH 5MM MGCL2 AND 2MM IDP PRIOR SETTING-UP THE PLATES. THEN IT WAS CRYSTALLIZED FROM 20MM MGCL2, 22% W/V POLYACRYLIC ACID 5100 AND 0.1M HEPES PH 7.5. |
