2XSF
Crystal structure of the RRM domain of mouse Deleted in azoospermia- like
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.900, 53.900, 65.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.000 - 1.700 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bs9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.587 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.600 |
| Number of reflections | 12616 | |
| <I/σ(I)> | 21.9 | 4.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.8 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2.1 M LITHIUM SULPHATE, 50 MM HEPES PH 7.5, 5 % (V/V) GLYCEROL |
