2XS6
CRYSTAL STRUCTURE OF THE RHOGAP DOMAIN OF HUMAN PIK3R2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-17 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.661, 34.354, 71.317 |
| Unit cell angles | 90.00, 130.88, 90.00 |
Refinement procedure
| Resolution | 53.920 - 2.090 |
| R-factor | 0.1942 |
| Rwork | 0.191 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pbw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 2.210 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.050 | 0.210 |
| Number of reflections | 9968 | |
| <I/σ(I)> | 9.9 | 3.7 |
| Completeness [%] | 95.6 | 76.6 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 0.1 M NACL, 0.85 M TRI-SODIUM CITRATE DIHYDRATE AND 0.1 M SODIUM CACODYLATE PH 6.5. |
