2XR1
DIMERIC ARCHAEAL CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR WITH N-TERMINAL KH DOMAINS (KH-CPSF) FROM METHANOSARCINA MAZEI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.660, 97.720, 90.720 |
| Unit cell angles | 90.00, 98.26, 90.00 |
Refinement procedure
| Resolution | 38.430 - 2.590 |
| R-factor | 0.21203 |
| Rwork | 0.209 |
| R-free | 0.26678 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i7v |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.483 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.600 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.570 |
| Number of reflections | 41154 | |
| <I/σ(I)> | 13.59 | 2.09 |
| Completeness [%] | 98.9 | 95.8 |
| Redundancy | 3.43 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20%(W/V) PEG3350, 200MM SODIUM NITRATE, pH 8 |
