2XQT
Microscopic rotary mechanism of ion translocation in the Fo complex of ATP synthases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-09-26 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 91.470, 91.470, 258.120 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.030 - 2.200 |
R-factor | 0.188 |
Rwork | 0.186 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wie |
RMSD bond length | 0.013 |
RMSD bond angle | 1.494 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.300 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.150 | 0.770 |
Number of reflections | 34801 | |
<I/σ(I)> | 12.02 | 3.03 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 14.3 | 14.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.3 | PH 4.3 |