2XPZ

Structure of native yeast LTA4 hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	70.833, 98.096, 99.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.981 - 2.300
R-factor	0.193
Rwork	0.190
R-free	0.26910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MODEL NATIVE SCLTA4 P3221
RMSD bond length	0.008
RMSD bond angle	1.117
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.090	0.240
Number of reflections	29531
<I/σ(I)>	5.2	1.4
Completeness [%]	94.8	86
Redundancy	2.2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION			THE CRYSTALS ARE GROWN IN LIQUID-LIQUID DIFFUSION EXPERIMENTS USING MELTING POINT CAPILLARIES IN WHICH 5 UL PROTEIN (6 MG/ML) IN 10 MM TRIS PH 8.5 IS LAYERED ON 5 UL 14% PEG 8000 AND 100 MM TRIS PH 7.3.