2XPO
Crystal structure of a Spt6-Iws1(Spn1) complex from Encephalitozoon cuniculi, Form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 112.578, 112.578, 51.481 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 97.500 - 2.100 |
| R-factor | 0.19031 |
| Rwork | 0.188 |
| R-free | 0.23862 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xpl |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.639 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.290 |
| Number of reflections | 21935 | |
| <I/σ(I)> | 33.6 | 10.47 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.7 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M SODIUM ACETATE 18% PEG1500 0.05 M MGCL2 |
