2XPN
Crystal structure of a Spt6-Iws1(Spn1) complex from Encephalitozoon cuniculi, Form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 52.311, 52.311, 151.125 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.450 - 1.950 |
| R-factor | 0.21112 |
| Rwork | 0.210 |
| R-free | 0.23486 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xpl |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.494 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.040 | 0.170 |
| Number of reflections | 16077 | |
| <I/σ(I)> | 78.8 | 17 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 12.4 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 22% PEG3350, 0.2 M NABR |
