2XPI
Crystal structure of APC/C hetero-tetramer Cut9-Hcn1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.400, 151.920, 91.870 |
| Unit cell angles | 90.00, 90.84, 90.00 |
Refinement procedure
| Resolution | 54.394 - 2.600 |
| R-factor | 0.1908 |
| Rwork | 0.189 |
| R-free | 0.24520 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.247 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.610 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 0.680 |
| Number of reflections | 45418 | |
| <I/σ(I)> | 9.1 | 2.5 |
| Completeness [%] | 99.2 | 96.4 |
| Redundancy | 5.5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPES (PH 7.5), 100 MM SODIUM ACETATE, 12.5% (W/V) PEG 3350 |
