2XOM
Atomic resolution structure of TmCBM61 in complex with beta-1,4- galactotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.915, 31.920, 67.790 |
| Unit cell angles | 90.00, 128.28, 90.00 |
Refinement procedure
| Resolution | 17.740 - 0.950 |
| R-factor | 0.10458 |
| Rwork | 0.104 |
| R-free | 0.11970 |
| Structure solution method | DIRECT METHODS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.025 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.000 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.040 | 0.210 |
| Number of reflections | 77217 | |
| <I/σ(I)> | 26.6 | 5.4 |
| Completeness [%] | 92.5 | 68.4 |
| Redundancy | 6.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
