2XOK
Refined structure of yeast F1c10 ATPase complex to 3 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.352, 173.711, 137.889 |
| Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.010 |
| R-factor | 0.21066 |
| Rwork | 0.210 |
| R-free | 0.25335 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hld |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.368 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.160 |
| High resolution limit [Å] | 3.010 | 3.000 |
| Rmerge | 0.090 | 0.180 |
| Number of reflections | 93955 | |
| <I/σ(I)> | 4.1 | 3.9 |
| Completeness [%] | 74.8 | 41.5 |
| Redundancy | 1.8 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 0.1 M TRIS/CL PH8.0, 12% PEG 6000, 150 MM NACL, 1 MM AMP-PNP, 40 MICROM ADP, 1 MM DTT, 0.02% NAN3. MIXED 1:1 WITH PROTEIN SOLUTION UNDER PARAFFIN OIL IN MICROBATCH PLATE. |
