2XOF
Ribonucleotide reductase Y122NO2Y modified R2 subunit of E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 137.103, 137.103, 108.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 118.680 - 2.200 |
| R-factor | 0.1934 |
| Rwork | 0.192 |
| R-free | 0.22352 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1av8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.123 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.290 | 2.220 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.120 | 0.540 |
| Number of reflections | 59034 | |
| <I/σ(I)> | 9.59 | 2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.68 | 3.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.9 | VAPOR DIFFUSION USING 90% OF SATURATED NACL, PH 7.9 |
