2XO6

DEINOCOCCUS RADIODURANS ISDRA2 TRANSPOSASE Y132F MUTANT COMPLEXED WITH LEFT END RECOGNITION AND CLEAVAGE SITE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2008-05-02
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	50.308, 86.915, 128.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.1805
Rwork	0.180
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xm3
RMSD bond length	0.019
RMSD bond angle	1.957
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.090	0.190
Number of reflections	44818
<I/σ(I)>	13.4	6.91
Completeness [%]	99.8	99.1
Redundancy	7.44	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.8	292	COMPLEX BUFFER: 35MM TRIS-HCL PH 8.0 0.15M NACL, 10MM MGCL2, 0.5 MM TCEP. PRECIPITATING AGENT: 10MM CDCL2, 0.1M NA-ACETATE PH 4.8, 14% PEG4000. PROTEIN AT 9MG/ML IN COMPLEX BUFFER WAS MIXED WITH LE27 AND T5G. PROTEIN AND DNA OLIGONUCLEOTIDES WERE MIXED IN THE RATIO 1:1.1:1.3. EQUAL VOLUMES OF THIS COMPLEX AND PRECIPITATING AGENT WAS MIXED AT 19 C. CRYOPROTECTION IN 20% V/V GLYCEROL.