2XO5
RIBONUCLEOTIDE REDUCTASE Y731NH2Y MODIFIED R1 SUBUNIT OF E. COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-27 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 223.238, 223.238, 335.665 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 166.667 - 2.700 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x0x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.224 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.060 | 2.720 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.510 |
| Number of reflections | 87927 | |
| <I/σ(I)> | 13.11 | 2.88 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.62 | 5.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6. |
