2XNY
A fragment of streptococcal M1 protein in complex with human fibrinogen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 165.687, 165.687, 289.652 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 142.860 - 7.500 |
| R-factor | 0.423 |
| Rwork | 0.424 |
| R-free | 0.40800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e1i |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.800 | 7.700 |
| High resolution limit [Å] | 6.000 | 7.500 |
| Rmerge | 0.190 | 0.330 |
| Number of reflections | 5481 | |
| <I/σ(I)> | 7.7 | 1.8 |
| Completeness [%] | 92.1 | 56.3 |
| Redundancy | 4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.3 M AMMONIUM TARTRATE, 0.1 M MES, PH 6.25. |
