2XN0

Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM, PtCl4 derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-19
Detector	MARRESEARCH MAR165
Spacegroup name	P 21 21 2
Unit cell lengths	126.370, 149.580, 90.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.839 - 2.500
R-factor	0.1502
Rwork	0.148
R-free	0.20260
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	1.072
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.130	0.500
Number of reflections	114981
<I/σ(I)>	15.2	4.9
Completeness [%]	98.4	96.4
Redundancy	7.6	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		8-12% PEG8000, 0.1M IMIDAZOLE, 10% GLYCEROL., PH 7.5