2XMU
Copper chaperone Atx1 from Synechocystis PCC6803 (Cu2 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.243, 29.388, 38.702 |
| Unit cell angles | 91.69, 101.26, 118.62 |
Refinement procedure
| Resolution | 37.580 - 1.750 |
| R-factor | 0.26915 |
| Rwork | 0.267 |
| R-free | 0.31157 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmj |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.536 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.570 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 10938 | |
| <I/σ(I)> | 22 | 9.7 |
| Completeness [%] | 98.4 | 97.6 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 28 % (W/V) PEG 8000, 200 MM SODIUM ACETATE, 100 HEPES PH 7.5 | ||
| 1 | 7.5 | 28 % (W/V) PEG 8000, 200 MM SODIUM ACETATE, 100 HEPES PH 7.5 |
