2XMT
Copper chaperone Atx1 from Synechocystis PCC6803 (Cu1 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.090, 29.660, 38.430 |
| Unit cell angles | 100.89, 92.58, 117.91 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.16256 |
| Rwork | 0.160 |
| R-free | 0.20279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmj |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.494 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 15929 | |
| <I/σ(I)> | 18.2 | 3.9 |
| Completeness [%] | 90.3 | 88.1 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 28%(W/V) PEG 8000, 200MM SODIUM ACETATE, 100MM SODIUM CACODYLATE PH 7.5 |
