2XME
The X-ray structure of CTP:inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-13 |
Detector | ADSC CCD |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 86.030, 127.550, 141.480 |
Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
Resolution | 27.670 - 1.890 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xmh |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 142.490 | 1.990 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.130 | 0.490 |
Number of reflections | 219870 | |
<I/σ(I)> | 10.1 | 1.5 |
Completeness [%] | 90.3 | 83 |
Redundancy | 4.3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.8 M SODIUM MALONATE PH 6.5 . |