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2XMA

DEINOCOCCUS RADIODURANS ISDRA2 TRANSPOSASE RIGHT END DNA COMPLEX

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]95
Detector technologyIMAGE PLATE
Collection date2008-03-17
DetectorRIGAKU RAXIS IV
Spacegroup nameP 1 21 1
Unit cell lengths76.827, 89.288, 90.104
Unit cell angles90.00, 111.23, 90.00
Refinement procedure
Resolution30.000 - 2.300
R-factor0.2152
Rwork0.215
R-free0.23750
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2xm3
RMSD bond length0.006
RMSD bond angle2.772
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.370
High resolution limit [Å]2.3002.300
Number of reflections50130
<I/σ(I)>16.43.09
Completeness [%]99.699
Redundancy3.623.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
18COMLEX BUFFER: 35MM TRIS-HCL PH 8.O, 0.1 M NA-MALONATE 10MM MGCL2M 0.5 MM TCEP PRECIPITATING AGENT: 0.16 MM MGCL2, 0.1 M TRIS-HCL PH 7.9, 3.4M 1,6-HEXANEDIOL FROZEN IN LIQUID PROPANE W/O ADDITIONAL CRYOPROTECTION

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