2XMA
DEINOCOCCUS RADIODURANS ISDRA2 TRANSPOSASE RIGHT END DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-17 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.827, 89.288, 90.104 |
| Unit cell angles | 90.00, 111.23, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.2152 |
| Rwork | 0.215 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xm3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 2.772 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 50130 | |
| <I/σ(I)> | 16.4 | 3.09 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 3.62 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | COMLEX BUFFER: 35MM TRIS-HCL PH 8.O, 0.1 M NA-MALONATE 10MM MGCL2M 0.5 MM TCEP PRECIPITATING AGENT: 0.16 MM MGCL2, 0.1 M TRIS-HCL PH 7.9, 3.4M 1,6-HEXANEDIOL FROZEN IN LIQUID PROPANE W/O ADDITIONAL CRYOPROTECTION |
