2XM3

Deinococcus radiodurans ISDra2 Transposase Left end DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2007-09-14
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	104.583, 128.384, 140.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.2179
Rwork	0.218
R-free	0.24890
Structure solution method	SIRAS
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	4.099
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.370
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.830
Number of reflections	84171
<I/σ(I)>	15.9	2.41
Completeness [%]	99.5	97.6
Redundancy	6.84	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6	292	COMPLEX BUFFER: 25 MM TRIS/HCL PH 8.0, 0.15M NACL PRECIPITATING AGENT: 17% PEG 6000, 50 MM NAACETATE PH 4.6 10MG/ML COMPLEX MIXED WITH EQUAL AMOUNT OF PRECIPITATING AGENT AT 19 C, CRYOPROTECTED WITH 15-20% GLYCEROL