2XLO
Cytochrome c prime from Alcaligenes xylosoxidans: Ferrous R124E variant with bound NO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 54.110, 54.110, 181.336 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.880 - 1.240 |
| R-factor | 0.17558 |
| Rwork | 0.174 |
| R-free | 0.19960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.985 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | 1.180 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.040 | 0.330 |
| Number of reflections | 58038 | |
| <I/σ(I)> | 35 | 3 |
| Completeness [%] | 98.7 | 88.6 |
| Redundancy | 8.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE, HEPES PH 7.5 |
