2XL3
WDR5 IN COMPLEX WITH AN RBBP5 PEPTIDE AND HISTONE H3 PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2009-11-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.100, 81.320, 86.350 |
| Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
| Resolution | 41.688 - 2.700 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h13 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.300 | 2.900 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.460 |
| Number of reflections | 16657 | |
| <I/σ(I)> | 7.2 | 2.5 |
| Completeness [%] | 98.7 | 99 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2M K2SO4 20 % PEG 3350, pH 8.0 |
