2XKA
Crystal structure of a GTPyS-form protofilament of Bacillus thuringiensis serovar israelensis TubZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.060, 85.150, 632.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.23459 |
| Rwork | 0.232 |
| R-free | 0.28438 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.333 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.690 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 | 0.340 |
| Number of reflections | 52941 | |
| <I/σ(I)> | 7.4 | 3.6 |
| Completeness [%] | 93.2 | 71 |
| Redundancy | 3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | TRISCL PH 8.5 200 MM MGCL2 30% PEG 400 WV |
