2XJO

Crystal structure of Streptococcus suis Dpr with nickel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-10
Detector	MARRESEARCH MARMOSAIC
Spacegroup name	P 21 21 21
Unit cell lengths	104.856, 137.459, 141.729
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.17801
Rwork	0.176
R-free	0.22164
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.015
RMSD bond angle	1.394
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.4.0078)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.070	0.370
Number of reflections	118580
<I/σ(I)>	19.6	2.5
Completeness [%]	99.0	95.5
Redundancy	5.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		30 - 35 % (V/V) PEG 400, 0.2 M CACL2, 0.1 M HEPES-NAOH (PH 7.4)