2XJM

Crystal structure of Streptococcus suis Dpr with cobalt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-10
Detector	MARRESEARCH MARMOSAIC
Spacegroup name	P 21 21 21
Unit cell lengths	104.960, 137.420, 141.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.910 - 2.300
R-factor	0.17986
Rwork	0.177
R-free	0.24030
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.014
RMSD bond angle	1.289
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.4.0078)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.050	0.110
Number of reflections	88486
<I/σ(I)>	16.6	7.4
Completeness [%]	96.2	76.9
Redundancy	3.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		30 - 35 % (V/V) PEG 400, 0.2 M CACL2, 0.1 M HEPES-NAOH (PH 7.4)