2XJK

Monomeric Human Cu,Zn Superoxide dismutase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-5
Synchrotron site	MAX II
Beamline	I911-5
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-13
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	35.160, 49.480, 80.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.450
R-factor	0.16788
Rwork	0.166
R-free	0.19944
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mfm
RMSD bond length	0.009
RMSD bond angle	1.201
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.490
High resolution limit [Å]	1.450	1.450
Rmerge	0.070	0.660
Number of reflections	25245
<I/σ(I)>	14	2.8
Completeness [%]	98.6	99.2
Redundancy	7.5	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		25% PEG 6000, 0.1M MES (PH 6.0), 0.01M ZNCL2