2XJE
Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II in complex with uridine 5'-monophosphate and adenosine triphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-10 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 91.650, 127.610, 130.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.090 - 2.300 |
| R-factor | 0.21749 |
| Rwork | 0.215 |
| R-free | 0.26905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jcm |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.785 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.410 |
| Number of reflections | 34194 | |
| <I/σ(I)> | 13.2 | 3.9 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BICINE PH 9, 10% PEG6000 |
