2XIG
The structure of the Helicobacter pylori ferric uptake regulator Fur reveals three functional metal binding sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.150, 48.290, 72.250 |
| Unit cell angles | 83.32, 77.82, 87.65 |
Refinement procedure
| Resolution | 35.097 - 1.850 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.23390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ZN-SAD MODEL AT 2.15A |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.870 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.960 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.100 | 0.390 |
| Number of reflections | 51362 | |
| <I/σ(I)> | 4.5 | 1.8 |
| Completeness [%] | 95.2 | 94.8 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | HPFUR2M PRE-LOADED WITH ZINC (2 EQ) AT 30 MG/ML WAS CRYSTALLIZED FROM 20% PEG 3350, 100 MM NA CITRATE, pH 7.4 |
