2XHU
HCV-J4 NS5B Polymerase Orthorhombic Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.520, 107.820, 133.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.101 - 2.287 |
| R-factor | 0.1679 |
| Rwork | 0.165 |
| R-free | 0.21770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nb4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.290 |
| Rmerge | 0.150 | 0.550 |
| Number of reflections | 69005 | |
| <I/σ(I)> | 12.9 | 2.36 |
| Completeness [%] | 98.2 | 97.1 |
| Redundancy | 5.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | MAGNESIUM SULFATE 0.2M, PEG 2000 MME 15%-25%, pH 7 |
