2XHK

Crystal structure of transcription factor NtcA from Synechococcus elongatus bound to 2-oxoglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-27
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	68.220, 70.810, 149.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.20566
Rwork	0.204
R-free	0.23769
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xgx
RMSD bond length	0.015
RMSD bond angle	1.563
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.050	0.440
Number of reflections	32971
<I/σ(I)>	13.3	1.8
Completeness [%]	99.8	100
Redundancy	7.1	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		NTCA PROTEIN WAS AT 4.7 MG/ML IN 50 MM SODIUM CITRATE PH 6.5, 0.5 M NACL, 5 MM MAGNESIUM CHLORIDE, 50 MM ARGININE HYDROCHLORIDE, 50 MM NA L-GLUTAMATE, 10 MM 2-OXOGLUTARATE (2OG). CRYSTALLIZATION SOLUTION: 0.1 M BIS-TRIS PH 5.5, 11 % PEG 10000, 0.15 M AMMONIUM ACETATE