2XHF

Crystal structure of peroxiredoxin 5 from Alvinella pompejana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-09
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	39.540, 92.920, 49.340
Unit cell angles	90.00, 109.55, 90.00

Refinement procedure

Resolution	22.182 - 1.300
R-factor	0.1517
Rwork	0.150
R-free	0.18410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hd2
RMSD bond length	0.010
RMSD bond angle	1.270
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.200	1.500
High resolution limit [Å]	1.300	1.300
Rmerge	0.060	0.390
Number of reflections	81682
<I/σ(I)>	13	3.4
Completeness [%]	99.2	99.4
Redundancy	3.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		HANGING DROP RESERVOIR: PEG 14000 17%(W/V), DTT 0.001M, MES PH 6.5 0.1M