2XHE
Crystal structure of the Unc18-syntaxin 1 complex from Monosiga brevicollis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 146.200, 146.200, 214.861 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.600 - 2.800 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c98 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.069 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.750 |
| Number of reflections | 34089 | |
| <I/σ(I)> | 19.8 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.7 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 7.5% PEG-6000, 0.1 M TRIS-HCL PH 7.0, 4.25% MPD, 15% GLYCEROL |
