2XHB
Crystal structure of DNA polymerase from Thermococcus gorgonarius in complex with hypoxanthine-containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.450, 98.370, 116.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.010 - 2.720 |
| R-factor | 0.23676 |
| Rwork | 0.234 |
| R-free | 0.29455 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vwj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.959 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.870 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.100 | 0.250 |
| Number of reflections | 23740 | |
| <I/σ(I)> | 8.8 | 4.2 |
| Completeness [%] | 95.1 | 97 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1M BICINE PH 9.0, 20% PEG6000 |
