2XH7
Engineering the enolase active site pocket: Crystal structure of the D321A mutant of yeast enolase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-07-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.973, 62.000, 64.320 |
| Unit cell angles | 67.89, 78.33, 80.60 |
Refinement procedure
| Resolution | 43.690 - 1.800 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xh4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.071 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.150 | 0.680 |
| Number of reflections | 73622 | |
| <I/σ(I)> | 11.43 | 3.12 |
| Completeness [%] | 91.3 | 89.6 |
| Redundancy | 1.81 | 1.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 18% PEG 6000, 0.2M MAGNESIUM CHLORIDE, 0.1M TRIS PH 8.0 |
