2XGQ
Structure of yeast DNA polymerase eta in complex with C8-N-acetyl-2- aminoanthracene containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-02-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 103.525, 103.525, 292.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.300 - 2.700 |
| R-factor | 0.21951 |
| Rwork | 0.217 |
| R-free | 0.26366 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wtf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.418 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 44732 | |
| <I/σ(I)> | 23 | 6 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 4MG/ML,11-14% PEG3350, 0.15-0.2M CACL2, 0.1M CTP, pH 7.6 |
