2XGP
Yeast DNA polymerase eta in complex with C8-2-acetylaminofluorene containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 103.440, 103.440, 292.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.760 - 2.700 |
| R-factor | 0.224 |
| Rwork | 0.220 |
| R-free | 0.27219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jih |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.442 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.520 |
| Number of reflections | 44372 | |
| <I/σ(I)> | 26.84 | 5.99 |
| Completeness [%] | 98.2 | 96.5 |
| Redundancy | 13.1 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4MG/ML, 11-14% PEG3350, 0.15-0.2M CACL2 |
