2XGD

Crystal structure of a designed homodimeric variant T-A(L)A(L) of the tetracycline repressor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Detector	RAYONIX
Spacegroup name	I 41 2 2
Unit cell lengths	69.656, 69.656, 184.328
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.840 - 2.250
R-factor	0.22032
Rwork	0.217
R-free	0.25886
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a6i
RMSD bond length	0.014
RMSD bond angle	1.445
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.390
High resolution limit [Å]	2.250	2.250
Rmerge	0.060	0.690
Number of reflections	11142
<I/σ(I)>	28.2	4.2
Completeness [%]	99.3	96.2
Redundancy	19.4	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1