2XFG
Reassembly and co-crystallization of a family 9 processive endoglucanase from separately expressed GH9 and CBM3c modules
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.399, 88.541, 106.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.868 - 1.679 |
| R-factor | 0.1432 |
| Rwork | 0.141 |
| R-free | 0.17590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g87 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.371 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.810 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 76727 | |
| <I/σ(I)> | 34.72 | 2.08 |
| Completeness [%] | 97.9 | 74.8 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 24% PEG 3350, 0.2 M MAGNESIUM CHLORIDE, 0.1 M HEPES, PH 8.0 |
