2XE5
Molecular insights into clinically isolated OmpC mutants and their role in multi-drug resistance (OmpC26)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.180, 93.680, 115.910 |
| Unit cell angles | 98.74, 108.79, 109.71 |
Refinement procedure
| Resolution | 29.410 - 2.280 |
| R-factor | 0.22804 |
| Rwork | 0.226 |
| R-free | 0.26707 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xe1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.361 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0060) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.000 | 2.340 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.080 | 0.450 |
| Number of reflections | 118130 | |
| <I/σ(I)> | 9.9 | 1.8 |
| Completeness [%] | 97.0 | 96 |
| Redundancy | 2.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
